This link’s a bit old, but I just love the Freudian theory here. Heroine Sheik makes the case that Portal is a feminist/lesbian game:
We’re dealing with a reshaping of a highly masculine genre, the FPS. If that itself weren’t “queering” enough, there’s the whole holes issue. We’ve talked before about how the guns in first-person shooters act as phallic avatars—that is, as penises. But in a world of women, this gun doesn’t shoot bullets. It shoots orifices. Openings. Fine, vaginas. Vaginas you, a female character, have to enter/exit to solve puzzles. I don’t say this often, and almost never with so much support and enthusiasm, but that is so gay.
Stuff like this makes me so damn thankful I went to a liberal arts college.